ssr_primer
Primer Details for TRINITY_DN75086_c9_g3_i1 |
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| (A)10taaataaaattttcagacaaagtcgtgaaaaaatcctgtg(A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCACGGATGGGAGAATCG | AAAACCTATCGGCTCCCTGC | Start: 2314 |
| Tm | 59.967 | 60.107 | End: 2463 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
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| Sequence | TCTCGGCGTTCACTGGAATC | AGCTTTTCGCCTCTCACGAA | Start: 1548 |
| Tm | 60.109 | 59.966 | End: 1667 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | TCTCGGCGTTCACTGGAATC | AAGCTTTTCGCCTCTCACGA | Start: 1548 |
| Tm | 60.109 | 59.966 | End: 1668 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
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| Sequence | GGATCGGAGGTCATGGCAAA | CCATCCTGGAAACGGTCACA | Start: 1295 |
| Tm | 60.107 | 59.964 | End: 1440 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | GCGGCACATTGGCATACAAA | CCATCCTGGAAACGGTCACA | Start: 1177 |
| Tm | 60.109 | 59.964 | End: 1440 |
| Size | 20 | 20 | 264 |
(C)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCACGGATGGGAGAATCG | AAAACCTATCGGCTCCCTGC | Start: 2314 |
| Tm | 59.967 | 60.107 | End: 2463 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
|---|
| Sequence | TCTCGGCGTTCACTGGAATC | AGCTTTTCGCCTCTCACGAA | Start: 1548 |
| Tm | 60.109 | 59.966 | End: 1667 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | TCTCGGCGTTCACTGGAATC | AAGCTTTTCGCCTCTCACGA | Start: 1548 |
| Tm | 60.109 | 59.966 | End: 1668 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | GGATCGGAGGTCATGGCAAA | CCATCCTGGAAACGGTCACA | Start: 1295 |
| Tm | 60.107 | 59.964 | End: 1440 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | GCGGCACATTGGCATACAAA | CCATCCTGGAAACGGTCACA | Start: 1177 |
| Tm | 60.109 | 59.964 | End: 1440 |
| Size | 20 | 20 | 264 |
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