ssr_primer
Primer Details for TRINITY_DN75036_c5_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTGGACAAGCTAGCTTCG | TTGGTTGGTGATGAGGGTCG | Start: 164 |
| Tm | 60.037 | 59.964 | End: 438 |
| Size | 20 | 20 | 275 |
| Primer 2 details |
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| Sequence | CTCGATGAGGTGGTGCTCTC | TTGGTTGGTGATGAGGGTCG | Start: 301 |
| Tm | 59.898 | 59.964 | End: 438 |
| Size | 20 | 20 | 138 |
| Primer 3 details |
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| Sequence | CCGGGAGATTCCTCGTCAAG | TTGGTTGGTGATGAGGGTCG | Start: 257 |
| Tm | 59.897 | 59.964 | End: 438 |
| Size | 20 | 20 | 182 |
| Primer 4 details |
|---|
| Sequence | TGGTGGACAAGCTAGCTTCG | GAGAGCACCACCTCATCGAG | Start: 164 |
| Tm | 60.037 | 59.898 | End: 320 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
|---|
| Sequence | TGGTGGACAAGCTAGCTTCG | CTTGACGAGGAATCTCCCGG | Start: 164 |
| Tm | 60.037 | 59.897 | End: 276 |
| Size | 20 | 20 | 113 |
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