ssr_primer
Primer Details for TRINITY_DN75009_c3_g2_i1 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGGCCTTCAAAAAGATGC | ATTGCGGTTCCTTCTCAGCA | Start: 1275 |
| Tm | 60.038 | 59.963 | End: 1389 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | TGCGGCCTTCAAAAAGATGC | TGCGGTTCCTTCTCAGCAAA | Start: 1275 |
| Tm | 60.038 | 60.179 | End: 1387 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | TGCGGCCTTCAAAAAGATGC | TTGCGGTTCCTTCTCAGCAA | Start: 1275 |
| Tm | 60.038 | 60.179 | End: 1388 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | CGGCCTTCAAAAAGATGCGA | ATTGCGGTTCCTTCTCAGCA | Start: 1277 |
| Tm | 59.479 | 59.963 | End: 1389 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | CCGGTGACTGGAGTTCAGAC | TACGTTCCCAGGATTTCGCA | Start: 32 |
| Tm | 60.038 | 59.391 | End: 136 |
| Size | 20 | 20 | 105 |
(A)10taaataaaaataaaatgaaactttcgtagtgaaattg(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCGGCCTTCAAAAAGATGC | ATTGCGGTTCCTTCTCAGCA | Start: 1275 |
| Tm | 60.038 | 59.963 | End: 1389 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | TGCGGCCTTCAAAAAGATGC | TGCGGTTCCTTCTCAGCAAA | Start: 1275 |
| Tm | 60.038 | 60.179 | End: 1387 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | TGCGGCCTTCAAAAAGATGC | TTGCGGTTCCTTCTCAGCAA | Start: 1275 |
| Tm | 60.038 | 60.179 | End: 1388 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | CGGCCTTCAAAAAGATGCGA | ATTGCGGTTCCTTCTCAGCA | Start: 1277 |
| Tm | 59.479 | 59.963 | End: 1389 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | CCGGTGACTGGAGTTCAGAC | TACGTTCCCAGGATTTCGCA | Start: 32 |
| Tm | 60.038 | 59.391 | End: 136 |
| Size | 20 | 20 | 105 |
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