ssr_primer
Primer Details for TRINITY_DN74989_c0_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGATCCCGTCCCATGTATG | ACGGGGTTCAGTGGTTTTGA | Start: 244 |
| Tm | 60.037 | 59.744 | End: 478 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
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| Sequence | CTCATCGCCGGGAGAACATT | TGGCGCAAGTTTAACCCTGA | Start: 34 |
| Tm | 60.179 | 60.179 | End: 167 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | TCATCGCCGGGAGAACATTT | TGGCGCAAGTTTAACCCTGA | Start: 35 |
| Tm | 59.748 | 60.179 | End: 167 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
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| Sequence | CGGATCCCGTCCCATGTATG | GGGACAGGAATAAACGGGGT | Start: 244 |
| Tm | 60.037 | 59.378 | End: 491 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
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| Sequence | CGGATCCCGTCCCATGTATG | GAAGCACCGCAACAAAGAGT | Start: 244 |
| Tm | 60.037 | 59.337 | End: 518 |
| Size | 20 | 20 | 275 |
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