ssr_primer
Primer Details for TRINITY_DN74987_c2_g1_i1 |
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| (G)21tggggtgtggggggggt(G)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAACTTGCGCCTGCTCATTT | CAGGCTGGCAGTGACTTACA | Start: 314 |
| Tm | 60.039 | 59.965 | End: 440 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
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| Sequence | CAACTTGCGCCTGCTCATTT | ACATCTCCGTGGGTTTTGCT | Start: 314 |
| Tm | 60.039 | 59.89 | End: 423 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
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| Sequence | GGAGCGCGAAACAATGGAAG | CAAAGAGTTCGCCCGCAAAA | Start: 856 |
| Tm | 60.179 | 59.97 | End: 969 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
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| Sequence | CCTTCCAAGCTCCGCGAATA | CAGGCTGGCAGTGACTTACA | Start: 268 |
| Tm | 60.179 | 59.965 | End: 440 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
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| Sequence | ACCAATCGAAATGCCTCCGA | CAAAGAGTTCGCCCGCAAAA | Start: 807 |
| Tm | 59.748 | 59.97 | End: 969 |
| Size | 20 | 20 | 163 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAACTTGCGCCTGCTCATTT | CAGGCTGGCAGTGACTTACA | Start: 314 |
| Tm | 60.039 | 59.965 | End: 440 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | CAACTTGCGCCTGCTCATTT | ACATCTCCGTGGGTTTTGCT | Start: 314 |
| Tm | 60.039 | 59.89 | End: 423 |
| Size | 20 | 20 | 110 |
| Primer 3 details |
|---|
| Sequence | GGAGCGCGAAACAATGGAAG | CAAAGAGTTCGCCCGCAAAA | Start: 856 |
| Tm | 60.179 | 59.97 | End: 969 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | CCTTCCAAGCTCCGCGAATA | CAGGCTGGCAGTGACTTACA | Start: 268 |
| Tm | 60.179 | 59.965 | End: 440 |
| Size | 20 | 20 | 173 |
| Primer 5 details |
|---|
| Sequence | ACCAATCGAAATGCCTCCGA | CAAAGAGTTCGCCCGCAAAA | Start: 807 |
| Tm | 59.748 | 59.97 | End: 969 |
| Size | 20 | 20 | 163 |
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