ssr_primer
Primer Details for TRINITY_DN74954_c5_g2_i1 |
|---|
| (G)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACAAGGCGGGTGAGTACA | TGGAACACCCAGCACGATTT | Start: 60 |
| Tm | 59.966 | 60.179 | End: 330 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | CAAGGCGGGTGAGTACAGTT | TGGAACACCCAGCACGATTT | Start: 63 |
| Tm | 59.965 | 60.179 | End: 330 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | AACAAGGCGGGTGAGTACAG | TGGAACACCCAGCACGATTT | Start: 61 |
| Tm | 59.965 | 60.179 | End: 330 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | AGAACAAGGCGGGTGAGTAC | TGGAACACCCAGCACGATTT | Start: 59 |
| Tm | 59.679 | 60.179 | End: 330 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | CGTGCTGGGTGTTCCAAAAG | AGTTAGGGTCCACAGTCCCC | Start: 315 |
| Tm | 59.969 | 60.547 | End: 453 |
| Size | 20 | 20 | 139 |
(C)11gaaaaaaaatcccacgcgga(G)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACAAGGCGGGTGAGTACA | TGGAACACCCAGCACGATTT | Start: 60 |
| Tm | 59.966 | 60.179 | End: 330 |
| Size | 20 | 20 | 271 |
| Primer 2 details |
|---|
| Sequence | CAAGGCGGGTGAGTACAGTT | TGGAACACCCAGCACGATTT | Start: 63 |
| Tm | 59.965 | 60.179 | End: 330 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | AACAAGGCGGGTGAGTACAG | TGGAACACCCAGCACGATTT | Start: 61 |
| Tm | 59.965 | 60.179 | End: 330 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
|---|
| Sequence | AGAACAAGGCGGGTGAGTAC | TGGAACACCCAGCACGATTT | Start: 59 |
| Tm | 59.679 | 60.179 | End: 330 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | CGTGCTGGGTGTTCCAAAAG | AGTTAGGGTCCACAGTCCCC | Start: 315 |
| Tm | 59.969 | 60.547 | End: 453 |
| Size | 20 | 20 | 139 |
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