ssr_primer
Primer Details for TRINITY_DN74948_c3_g3_i1 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGCACTTACATGGGCGGA | TCGAACCTTTACGACGGCAA | Start: 363 |
| Tm | 59.962 | 59.968 | End: 532 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
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| Sequence | AAAGCACTTACATGGGCGGA | TTCGAACCTTTACGACGGCA | Start: 363 |
| Tm | 59.962 | 59.968 | End: 533 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
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| Sequence | ATTGCTGAGTGGTGATGGGG | TCCGCCCATGTAAGTGCTTT | Start: 132 |
| Tm | 60.034 | 59.962 | End: 382 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
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| Sequence | GGCATGCGTGGTTTTAGCAA | CCCCATCACCACTCAGCAAT | Start: 35 |
| Tm | 60.039 | 60.034 | End: 151 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
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| Sequence | AGGAGGTCGTCAGAATGGGA | TCGAACCTTTACGACGGCAA | Start: 274 |
| Tm | 59.959 | 59.968 | End: 532 |
| Size | 20 | 20 | 259 |
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