ssr_primer
Primer Details for TRINITY_DN74930_c4_g1_i4 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGTTCCCTGCCATCCAAA | TTGACCTACCGCACAACTCC | Start: 4464 |
| Tm | 59.96 | 59.966 | End: 4659 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | TCCGTGCATCGTTTTCTCCA | GTGCGGTGTTTGCGGTTAAT | Start: 797 |
| Tm | 59.966 | 60.04 | End: 1009 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
|---|
| Sequence | TCCGTGCATCGTTTTCTCCA | TGCGGTGTTTGCGGTTAATG | Start: 797 |
| Tm | 59.966 | 60.04 | End: 1008 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
|---|
| Sequence | ACTCTTGCTGTCTGACGAGC | TTTGGATGGCAGGGAACTCC | Start: 4300 |
| Tm | 60.039 | 59.96 | End: 4483 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | GGAGTTCCCTGCCATCCAAA | CGGAAGACCTCCTCCTCGTA | Start: 4464 |
| Tm | 59.96 | 60.107 | End: 4711 |
| Size | 20 | 20 | 248 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGTTCCCTGCCATCCAAA | TTGACCTACCGCACAACTCC | Start: 4464 |
| Tm | 59.96 | 59.966 | End: 4659 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | TCCGTGCATCGTTTTCTCCA | GTGCGGTGTTTGCGGTTAAT | Start: 797 |
| Tm | 59.966 | 60.04 | End: 1009 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
|---|
| Sequence | TCCGTGCATCGTTTTCTCCA | TGCGGTGTTTGCGGTTAATG | Start: 797 |
| Tm | 59.966 | 60.04 | End: 1008 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
|---|
| Sequence | ACTCTTGCTGTCTGACGAGC | TTTGGATGGCAGGGAACTCC | Start: 4300 |
| Tm | 60.039 | 59.96 | End: 4483 |
| Size | 20 | 20 | 184 |
| Primer 5 details |
|---|
| Sequence | GGAGTTCCCTGCCATCCAAA | CGGAAGACCTCCTCCTCGTA | Start: 4464 |
| Tm | 59.96 | 60.107 | End: 4711 |
| Size | 20 | 20 | 248 |
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