ssr_primer
Primer Details for TRINITY_DN74899_c3_g5_i2 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATAAAGCGCGCATCGTGAC | CTTCAATCGCAGCTCTTGGC | Start: 20 |
| Tm | 59.974 | 59.902 | End: 168 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
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| Sequence | GCCAAGAGCTGCGATTGAAG | GTATGGGGCCGGTAAACACA | Start: 149 |
| Tm | 59.902 | 60.035 | End: 335 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
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| Sequence | GCCAAGAGCTGCGATTGAAG | TGTATGGGGCCGGTAAACAC | Start: 149 |
| Tm | 59.902 | 60.035 | End: 336 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | AAATTGAAGGCGCGATGGTG | GTATGGGGCCGGTAAACACA | Start: 92 |
| Tm | 59.829 | 60.035 | End: 335 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | AAATTGAAGGCGCGATGGTG | TGTATGGGGCCGGTAAACAC | Start: 92 |
| Tm | 59.829 | 60.035 | End: 336 |
| Size | 20 | 20 | 245 |
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