ssr_primer
Primer Details for TRINITY_DN74852_c0_g1_i1 |
|---|
| (T)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTGGCGGTGTCTTGATCT | AGCTTGAAGTGATGGTGGGG | Start: 480 |
| Tm | 60.037 | 59.961 | End: 620 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | ACTGCCTCACGCTCAATCAA | GCTGCAGTTACGCTCTTTCG | Start: 207 |
| Tm | 59.965 | 59.906 | End: 369 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | CGAAAGAGCGTAACTGCAGC | AGATCAAGACACCGCCACAG | Start: 350 |
| Tm | 59.906 | 60.037 | End: 499 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | CGAAAGAGCGTAACTGCAGC | AGCTTGAAGTGATGGTGGGG | Start: 350 |
| Tm | 59.906 | 59.961 | End: 620 |
| Size | 20 | 20 | 271 |
| Primer 5 details |
|---|
| Sequence | CTGTGGCGGTGTCTTGATCT | GGATGATTCAAAAGCGCGCT | Start: 480 |
| Tm | 60.037 | 59.901 | End: 580 |
| Size | 20 | 20 | 101 |
(C)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTGGCGGTGTCTTGATCT | AGCTTGAAGTGATGGTGGGG | Start: 480 |
| Tm | 60.037 | 59.961 | End: 620 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | ACTGCCTCACGCTCAATCAA | GCTGCAGTTACGCTCTTTCG | Start: 207 |
| Tm | 59.965 | 59.906 | End: 369 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | CGAAAGAGCGTAACTGCAGC | AGATCAAGACACCGCCACAG | Start: 350 |
| Tm | 59.906 | 60.037 | End: 499 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | CGAAAGAGCGTAACTGCAGC | AGCTTGAAGTGATGGTGGGG | Start: 350 |
| Tm | 59.906 | 59.961 | End: 620 |
| Size | 20 | 20 | 271 |
| Primer 5 details |
|---|
| Sequence | CTGTGGCGGTGTCTTGATCT | GGATGATTCAAAAGCGCGCT | Start: 480 |
| Tm | 60.037 | 59.901 | End: 580 |
| Size | 20 | 20 | 101 |
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