ssr_primer
Primer Details for TRINITY_DN74837_c6_g1_i17 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGATTTGAGTCCGGCCTG | GGTATCAAACACGTACGCGC | Start: 198 |
| Tm | 60.036 | 59.976 | End: 438 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
|---|
| Sequence | TGGAAGGCAAAGGGGTGTTT | CAGGCCGGACTCAAATCAGT | Start: 83 |
| Tm | 60.032 | 60.036 | End: 217 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | CAACTCCTCAGACAGGCTGG | ATGGCCTGTTTTCGTGTTGC | Start: 1291 |
| Tm | 60.037 | 59.968 | End: 1505 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | CAACTCCTCAGACAGGCTGG | TGTTGCAGGTGAGCTCGAAT | Start: 1291 |
| Tm | 60.037 | 59.965 | End: 1491 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TTTCGCGATGCTCGACCATA | GGTATCAAACACGTACGCGC | Start: 258 |
| Tm | 59.898 | 59.976 | End: 438 |
| Size | 20 | 20 | 181 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGATTTGAGTCCGGCCTG | GGTATCAAACACGTACGCGC | Start: 198 |
| Tm | 60.036 | 59.976 | End: 438 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
|---|
| Sequence | TGGAAGGCAAAGGGGTGTTT | CAGGCCGGACTCAAATCAGT | Start: 83 |
| Tm | 60.032 | 60.036 | End: 217 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | CAACTCCTCAGACAGGCTGG | ATGGCCTGTTTTCGTGTTGC | Start: 1291 |
| Tm | 60.037 | 59.968 | End: 1505 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | CAACTCCTCAGACAGGCTGG | TGTTGCAGGTGAGCTCGAAT | Start: 1291 |
| Tm | 60.037 | 59.965 | End: 1491 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TTTCGCGATGCTCGACCATA | GGTATCAAACACGTACGCGC | Start: 258 |
| Tm | 59.898 | 59.976 | End: 438 |
| Size | 20 | 20 | 181 |
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