ssr_primer
Primer Details for TRINITY_DN74821_c3_g3_i1 |
|---|
| (G)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTATGCATTGCTCGTCGCG | TAGACAAATGGCGCCAGTGT | Start: 1227 |
| Tm | 59.974 | 59.964 | End: 1452 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | TTTCGGTCATCGGTCTTGGG | GTAAGCGGGGTGTCAAAGGA | Start: 100 |
| Tm | 60.037 | 59.964 | End: 310 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | ACACTGGCGCCATTTGTCTA | GGAGGAAGCAAGCTCCAGTT | Start: 1433 |
| Tm | 59.964 | 59.963 | End: 1639 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | ACACTGGCGCCATTTGTCTA | TGAGTACTGCCTGCGAAGTG | Start: 1433 |
| Tm | 59.964 | 60.038 | End: 1554 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | CACTTCGCAGGCAGTACTCA | GGAGGAAGCAAGCTCCAGTT | Start: 1535 |
| Tm | 60.038 | 59.963 | End: 1639 |
| Size | 20 | 20 | 105 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTATGCATTGCTCGTCGCG | TAGACAAATGGCGCCAGTGT | Start: 1227 |
| Tm | 59.974 | 59.964 | End: 1452 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | TTTCGGTCATCGGTCTTGGG | GTAAGCGGGGTGTCAAAGGA | Start: 100 |
| Tm | 60.037 | 59.964 | End: 310 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | ACACTGGCGCCATTTGTCTA | GGAGGAAGCAAGCTCCAGTT | Start: 1433 |
| Tm | 59.964 | 59.963 | End: 1639 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
|---|
| Sequence | ACACTGGCGCCATTTGTCTA | TGAGTACTGCCTGCGAAGTG | Start: 1433 |
| Tm | 59.964 | 60.038 | End: 1554 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | CACTTCGCAGGCAGTACTCA | GGAGGAAGCAAGCTCCAGTT | Start: 1535 |
| Tm | 60.038 | 59.963 | End: 1639 |
| Size | 20 | 20 | 105 |
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