ssr_primer
Primer Details for TRINITY_DN74769_c1_g1_i1 |
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| (A)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGACCCTGCAGCTTGTAAG | TGGCTTAGCTCCAAAGACGG | Start: 761 |
| Tm | 59.963 | 60.036 | End: 930 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
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| Sequence | CCGTCTTTGGAGCTAAGCCA | GTCTTGGTCAGTCCGTCTGG | Start: 911 |
| Tm | 60.036 | 60.038 | End: 1088 |
| Size | 20 | 20 | 178 |
| Primer 3 details |
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| Sequence | CCGTCTTTGGAGCTAAGCCA | TTGCTGCTCAAAACGATGCC | Start: 911 |
| Tm | 60.036 | 60.039 | End: 1046 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
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| Sequence | CCGTCTTTGGAGCTAAGCCA | GCGCTTTGTGTTTGTCCGAT | Start: 911 |
| Tm | 60.036 | 60.04 | End: 1180 |
| Size | 20 | 20 | 270 |
| Primer 5 details |
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| Sequence | GGCATCGTTTTGAGCAGCAA | GCGCTTTGTGTTTGTCCGAT | Start: 1027 |
| Tm | 60.039 | 60.04 | End: 1180 |
| Size | 20 | 20 | 154 |
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