Primer Details for TRINITY_DN74768_c4_g1_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAACCGAATAAACTAGCCAAAAA | TGAGGACCAGTTTTGTGCTGT | Start: 46 |
| Tm | 57.363 | 60.065 | End: 149 |
| Size | 24 | 21 | 104 |
| Primer 2 details | |||
| Sequence | TTCAACCGAATAAACTAGCCAAAA | TGAGGACCAGTTTTGTGCTGT | Start: 45 |
| Tm | 57.363 | 60.065 | End: 149 |
| Size | 24 | 21 | 105 |
| Primer 3 details | |||
| Sequence | ATTCAACCGAATAAACTAGCCAAA | TGAGGACCAGTTTTGTGCTGT | Start: 44 |
| Tm | 57.172 | 60.065 | End: 149 |
| Size | 24 | 21 | 106 |
| Primer 4 details | |||
| Sequence | TTCAACCGAATAAACTAGCCAAAAA | TGAGGACCAGTTTTGTGCTGT | Start: 45 |
| Tm | 57.96 | 60.065 | End: 149 |
| Size | 25 | 21 | 105 |
| Primer 5 details | |||
| Sequence | TCAACCGAATAAACTAGCCAAAAA | GGACCAGTTTTGTGCTGTACC | Start: 46 |
| Tm | 57.363 | 59.393 | End: 146 |
| Size | 24 | 21 | 101 | (T)10(A)10: Primer 1 details |
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAACCGAATAAACTAGCCAAAAA | TGAGGACCAGTTTTGTGCTGT | Start: 46 |
| Tm | 57.363 | 60.065 | End: 149 |
| Size | 24 | 21 | 104 |
| Primer 2 details | |||
| Sequence | TTCAACCGAATAAACTAGCCAAAA | TGAGGACCAGTTTTGTGCTGT | Start: 45 |
| Tm | 57.363 | 60.065 | End: 149 |
| Size | 24 | 21 | 105 |
| Primer 3 details | |||
| Sequence | ATTCAACCGAATAAACTAGCCAAA | TGAGGACCAGTTTTGTGCTGT | Start: 44 |
| Tm | 57.172 | 60.065 | End: 149 |
| Size | 24 | 21 | 106 |
| Primer 4 details | |||
| Sequence | TTCAACCGAATAAACTAGCCAAAAA | TGAGGACCAGTTTTGTGCTGT | Start: 45 |
| Tm | 57.96 | 60.065 | End: 149 |
| Size | 25 | 21 | 105 |
| Primer 5 details | |||
| Sequence | TCAACCGAATAAACTAGCCAAAAA | GGACCAGTTTTGTGCTGTACC | Start: 46 |
| Tm | 57.363 | 59.393 | End: 146 |
| Size | 24 | 21 | 101 |
