ssr_primer
Primer Details for TRINITY_DN74763_c2_g4_i5 |
|---|
| (A)43: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTTCGTGGCCCTACTCAC | AGGAAGTTTACGGCCCGTTT | Start: 2011 |
| Tm | 59.966 | 59.891 | End: 2230 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | CCTCTGATTGGGCGGGAAAT | CACAAAATTGCAGGCGAGCT | Start: 988 |
| Tm | 60.107 | 60.039 | End: 1112 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | GTAACCAAGTAGCCCGGGTC | GGTTGGGACGAGGGGAAAAT | Start: 631 |
| Tm | 60.108 | 59.961 | End: 745 |
| Size | 20 | 20 | 115 |
| Primer 4 details |
|---|
| Sequence | CCCTCAGGACGATGGTCAAC | GGTTGGGACGAGGGGAAAAT | Start: 478 |
| Tm | 60.108 | 59.961 | End: 745 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | CCGAACGGGAAGAAGTGACA | TTCACGATGATACCGCCGTT | Start: 1060 |
| Tm | 59.967 | 59.826 | End: 1291 |
| Size | 20 | 20 | 232 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTTCGTGGCCCTACTCAC | AGGAAGTTTACGGCCCGTTT | Start: 2011 |
| Tm | 59.966 | 59.891 | End: 2230 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | CCTCTGATTGGGCGGGAAAT | CACAAAATTGCAGGCGAGCT | Start: 988 |
| Tm | 60.107 | 60.039 | End: 1112 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | GTAACCAAGTAGCCCGGGTC | GGTTGGGACGAGGGGAAAAT | Start: 631 |
| Tm | 60.108 | 59.961 | End: 745 |
| Size | 20 | 20 | 115 |
| Primer 4 details |
|---|
| Sequence | CCCTCAGGACGATGGTCAAC | GGTTGGGACGAGGGGAAAAT | Start: 478 |
| Tm | 60.108 | 59.961 | End: 745 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | CCGAACGGGAAGAAGTGACA | TTCACGATGATACCGCCGTT | Start: 1060 |
| Tm | 59.967 | 59.826 | End: 1291 |
| Size | 20 | 20 | 232 |
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