ssr_primer
Primer Details for TRINITY_DN74742_c1_g1_i3 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCAATCCTCTGTCCGACGG | TGCATGTGCGTGTTGAATGG | Start: 100 |
| Tm | 59.899 | 60.039 | End: 345 |
| Size | 20 | 20 | 246 |
| Primer 2 details |
|---|
| Sequence | TACCCATACCCACACACCCT | TGGGAGAAAATGTCGACCGG | Start: 374 |
| Tm | 59.88 | 60.037 | End: 641 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | CCGGTCGACATTTTCTCCCA | AACGCTTCCTCGATTCTCCC | Start: 622 |
| Tm | 60.037 | 59.824 | End: 794 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TAACGTGTGCAAAAGTCGCG | AACGCTTCCTCGATTCTCCC | Start: 664 |
| Tm | 60.043 | 59.824 | End: 794 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | CGGGACACCTGAATTGCAAC | TGGGAGAAAATGTCGACCGG | Start: 448 |
| Tm | 59.759 | 60.037 | End: 641 |
| Size | 20 | 20 | 194 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCAATCCTCTGTCCGACGG | TGCATGTGCGTGTTGAATGG | Start: 100 |
| Tm | 59.899 | 60.039 | End: 345 |
| Size | 20 | 20 | 246 |
| Primer 2 details |
|---|
| Sequence | TACCCATACCCACACACCCT | TGGGAGAAAATGTCGACCGG | Start: 374 |
| Tm | 59.88 | 60.037 | End: 641 |
| Size | 20 | 20 | 268 |
| Primer 3 details |
|---|
| Sequence | CCGGTCGACATTTTCTCCCA | AACGCTTCCTCGATTCTCCC | Start: 622 |
| Tm | 60.037 | 59.824 | End: 794 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TAACGTGTGCAAAAGTCGCG | AACGCTTCCTCGATTCTCCC | Start: 664 |
| Tm | 60.043 | 59.824 | End: 794 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | CGGGACACCTGAATTGCAAC | TGGGAGAAAATGTCGACCGG | Start: 448 |
| Tm | 59.759 | 60.037 | End: 641 |
| Size | 20 | 20 | 194 |
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