ssr_primer
Primer Details for TRINITY_DN74716_c0_g1_i2 |
|---|
| (G)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTGACATTTCCCGGACAA | TCGTGTCAGGGAAACGTCAG | Start: 241 |
| Tm | 59.962 | 59.968 | End: 487 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TAGGTTGGCAAATGCAGGGT | TTGTCCGGGAAATGTCAGGG | Start: 114 |
| Tm | 59.886 | 59.962 | End: 260 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | CCCTGACATTTCCCGGACAA | CGACCATTACTCCTGCGGAA | Start: 241 |
| Tm | 59.962 | 59.825 | End: 429 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | CCCTGACATTTCCCGGACAA | CGTGTCAGGGAAACGTCAGT | Start: 241 |
| Tm | 59.962 | 60.249 | End: 486 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | GCAGGGTGTCTGGCAACTAA | TTGTCCGGGAAATGTCAGGG | Start: 127 |
| Tm | 60.251 | 59.962 | End: 260 |
| Size | 20 | 20 | 134 |
(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTGACATTTCCCGGACAA | TCGTGTCAGGGAAACGTCAG | Start: 241 |
| Tm | 59.962 | 59.968 | End: 487 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TAGGTTGGCAAATGCAGGGT | TTGTCCGGGAAATGTCAGGG | Start: 114 |
| Tm | 59.886 | 59.962 | End: 260 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | CCCTGACATTTCCCGGACAA | CGACCATTACTCCTGCGGAA | Start: 241 |
| Tm | 59.962 | 59.825 | End: 429 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | CCCTGACATTTCCCGGACAA | CGTGTCAGGGAAACGTCAGT | Start: 241 |
| Tm | 59.962 | 60.249 | End: 486 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | GCAGGGTGTCTGGCAACTAA | TTGTCCGGGAAATGTCAGGG | Start: 127 |
| Tm | 60.251 | 59.962 | End: 260 |
| Size | 20 | 20 | 134 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTGACATTTCCCGGACAA | TCGTGTCAGGGAAACGTCAG | Start: 241 |
| Tm | 59.962 | 59.968 | End: 487 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | TAGGTTGGCAAATGCAGGGT | TTGTCCGGGAAATGTCAGGG | Start: 114 |
| Tm | 59.886 | 59.962 | End: 260 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | CCCTGACATTTCCCGGACAA | CGACCATTACTCCTGCGGAA | Start: 241 |
| Tm | 59.962 | 59.825 | End: 429 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | CCCTGACATTTCCCGGACAA | CGTGTCAGGGAAACGTCAGT | Start: 241 |
| Tm | 59.962 | 60.249 | End: 486 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | GCAGGGTGTCTGGCAACTAA | TTGTCCGGGAAATGTCAGGG | Start: 127 |
| Tm | 60.251 | 59.962 | End: 260 |
| Size | 20 | 20 | 134 |
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