ssr_primer
Primer Details for TRINITY_DN74714_c1_g2_i8 |
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| (A)11tgatcaaatacaacagaaat(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCTGAACTCTCATGGGGC | TGTCCAATGAACCAGACGGG | Start: 2611 |
| Tm | 60.035 | 59.964 | End: 2876 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
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| Sequence | TGTCTGCGACATGTAACCCC | AAGATTGGGGCTGGCAAAGT | Start: 399 |
| Tm | 60.037 | 60.179 | End: 668 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
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| Sequence | AAGCTGAACTCTCATGGGGC | CGAGACAGCAGTGATGACCA | Start: 2611 |
| Tm | 60.035 | 59.755 | End: 2844 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | AAGCTGAACTCTCATGGGGC | ACGAGACAGCAGTGATGACC | Start: 2611 |
| Tm | 60.035 | 59.755 | End: 2845 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
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| Sequence | GCAGTCCAAATCAATGGCGG | CCTCAGCTACCGCTACCAAG | Start: 2344 |
| Tm | 60.179 | 59.897 | End: 2520 |
| Size | 20 | 20 | 177 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCTGAACTCTCATGGGGC | TGTCCAATGAACCAGACGGG | Start: 2611 |
| Tm | 60.035 | 59.964 | End: 2876 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | TGTCTGCGACATGTAACCCC | AAGATTGGGGCTGGCAAAGT | Start: 399 |
| Tm | 60.037 | 60.179 | End: 668 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
|---|
| Sequence | AAGCTGAACTCTCATGGGGC | CGAGACAGCAGTGATGACCA | Start: 2611 |
| Tm | 60.035 | 59.755 | End: 2844 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | AAGCTGAACTCTCATGGGGC | ACGAGACAGCAGTGATGACC | Start: 2611 |
| Tm | 60.035 | 59.755 | End: 2845 |
| Size | 20 | 20 | 235 |
| Primer 5 details |
|---|
| Sequence | GCAGTCCAAATCAATGGCGG | CCTCAGCTACCGCTACCAAG | Start: 2344 |
| Tm | 60.179 | 59.897 | End: 2520 |
| Size | 20 | 20 | 177 |
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