ssr_primer
Primer Details for TRINITY_DN74668_c2_g1_i2 |
|---|
| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGCTTTCATGTTCCGCAC | TTTCCAGGACGAGACTTGGC | Start: 326 |
| Tm | 60.11 | 59.965 | End: 444 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | GGCTTTCATGTTCCGCACTC | TTTCCAGGACGAGACTTGGC | Start: 328 |
| Tm | 59.831 | 59.965 | End: 444 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | ACTCTACATCTCCCCTCGCA | TTTCCAGGACGAGACTTGGC | Start: 344 |
| Tm | 59.741 | 59.965 | End: 444 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | ATCCGACAAAAAGGGGAGGC | TGTCGGAAGTCACTGACAGC | Start: 1613 |
| Tm | 60.323 | 59.968 | End: 1812 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
|---|
| Sequence | GCACGCAGTCTTTTTCGCAT | TCCTTGCGAGGGGAGATGTA | Start: 128 |
| Tm | 60.386 | 60.033 | End: 367 |
| Size | 20 | 20 | 240 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGCTTTCATGTTCCGCAC | TTTCCAGGACGAGACTTGGC | Start: 326 |
| Tm | 60.11 | 59.965 | End: 444 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | GGCTTTCATGTTCCGCACTC | TTTCCAGGACGAGACTTGGC | Start: 328 |
| Tm | 59.831 | 59.965 | End: 444 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | ACTCTACATCTCCCCTCGCA | TTTCCAGGACGAGACTTGGC | Start: 344 |
| Tm | 59.741 | 59.965 | End: 444 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | ATCCGACAAAAAGGGGAGGC | TGTCGGAAGTCACTGACAGC | Start: 1613 |
| Tm | 60.323 | 59.968 | End: 1812 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
|---|
| Sequence | GCACGCAGTCTTTTTCGCAT | TCCTTGCGAGGGGAGATGTA | Start: 128 |
| Tm | 60.386 | 60.033 | End: 367 |
| Size | 20 | 20 | 240 |
(T)37: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGCTTTCATGTTCCGCAC | TTTCCAGGACGAGACTTGGC | Start: 326 |
| Tm | 60.11 | 59.965 | End: 444 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | GGCTTTCATGTTCCGCACTC | TTTCCAGGACGAGACTTGGC | Start: 328 |
| Tm | 59.831 | 59.965 | End: 444 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | ACTCTACATCTCCCCTCGCA | TTTCCAGGACGAGACTTGGC | Start: 344 |
| Tm | 59.741 | 59.965 | End: 444 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
|---|
| Sequence | ATCCGACAAAAAGGGGAGGC | TGTCGGAAGTCACTGACAGC | Start: 1613 |
| Tm | 60.323 | 59.968 | End: 1812 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
|---|
| Sequence | GCACGCAGTCTTTTTCGCAT | TCCTTGCGAGGGGAGATGTA | Start: 128 |
| Tm | 60.386 | 60.033 | End: 367 |
| Size | 20 | 20 | 240 |
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