ssr_primer
Primer Details for TRINITY_DN74628_c7_g1_i1 |
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| (G)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGCTACGGACTTTTGGAA | GCGTCACCCCTAATCACACA | Start: 96 |
| Tm | 59.963 | 60.037 | End: 323 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
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| Sequence | AAAAGTTACACGGGTGGGGG | GCGTCACCCCTAATCACACA | Start: 74 |
| Tm | 60.179 | 60.037 | End: 323 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
|---|
| Sequence | TGTGTGATTAGGGGTGACGC | GCTCTTTGCTTCCACTCCCA | Start: 304 |
| Tm | 60.037 | 60.251 | End: 509 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
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| Sequence | TGTGTGATTAGGGGTGACGC | TTTGTGAGCACTAAGGGGCC | Start: 304 |
| Tm | 60.037 | 60.251 | End: 530 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
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| Sequence | TGGGAGTGGAAGCAAAGAGC | GATAATGGGCGTGGGGCTTA | Start: 490 |
| Tm | 60.251 | 59.89 | End: 709 |
| Size | 20 | 20 | 220 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGCTACGGACTTTTGGAA | GCGTCACCCCTAATCACACA | Start: 96 |
| Tm | 59.963 | 60.037 | End: 323 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | AAAAGTTACACGGGTGGGGG | GCGTCACCCCTAATCACACA | Start: 74 |
| Tm | 60.179 | 60.037 | End: 323 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
|---|
| Sequence | TGTGTGATTAGGGGTGACGC | GCTCTTTGCTTCCACTCCCA | Start: 304 |
| Tm | 60.037 | 60.251 | End: 509 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
|---|
| Sequence | TGTGTGATTAGGGGTGACGC | TTTGTGAGCACTAAGGGGCC | Start: 304 |
| Tm | 60.037 | 60.251 | End: 530 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
|---|
| Sequence | TGGGAGTGGAAGCAAAGAGC | GATAATGGGCGTGGGGCTTA | Start: 490 |
| Tm | 60.251 | 59.89 | End: 709 |
| Size | 20 | 20 | 220 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGCTACGGACTTTTGGAA | GCGTCACCCCTAATCACACA | Start: 96 |
| Tm | 59.963 | 60.037 | End: 323 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | AAAAGTTACACGGGTGGGGG | GCGTCACCCCTAATCACACA | Start: 74 |
| Tm | 60.179 | 60.037 | End: 323 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
|---|
| Sequence | TGTGTGATTAGGGGTGACGC | GCTCTTTGCTTCCACTCCCA | Start: 304 |
| Tm | 60.037 | 60.251 | End: 509 |
| Size | 20 | 20 | 206 |
| Primer 4 details |
|---|
| Sequence | TGTGTGATTAGGGGTGACGC | TTTGTGAGCACTAAGGGGCC | Start: 304 |
| Tm | 60.037 | 60.251 | End: 530 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
|---|
| Sequence | TGGGAGTGGAAGCAAAGAGC | GATAATGGGCGTGGGGCTTA | Start: 490 |
| Tm | 60.251 | 59.89 | End: 709 |
| Size | 20 | 20 | 220 |
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