ssr_primer
Primer Details for TRINITY_DN74621_c3_g2_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACTTTGGGTGGAGTGGACC | AGTTGAACGCACACTCTGCT | Start: 131 |
| Tm | 59.815 | 60.179 | End: 348 |
| Size | 20 | 20 | 218 |
| Primer 2 details |
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| Sequence | AACTTTGGGTGGAGTGGACC | GTTGAACGCACACTCTGCTT | Start: 131 |
| Tm | 59.815 | 59.342 | End: 347 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
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| Sequence | ACTTTGGGTGGAGTGGACCA | AGTTGAACGCACACTCTGCT | Start: 132 |
| Tm | 60.991 | 60.179 | End: 348 |
| Size | 20 | 20 | 217 |
| Primer 4 details |
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| Sequence | GGACCACTAGAAAGGTTGCGA | AGTTGAACGCACACTCTGCT | Start: 146 |
| Tm | 59.998 | 60.179 | End: 348 |
| Size | 21 | 20 | 203 |
| Primer 5 details |
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| Sequence | AGGTGTGTAGGCAGGGTATCT | GGTCCACTCCACCCAAAGTT | Start: 2 |
| Tm | 59.992 | 59.815 | End: 150 |
| Size | 21 | 20 | 149 |
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