ssr_primer
Primer Details for TRINITY_DN74589_c5_g2_i5 |
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| (T)13atctgaaggaagagtggtgaaaaaatcactattaacaatactaccatattattgcactgaacttaaatgaaaaagcaa(AG)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGCACCACACACCACTAT | GCTCTAAGGGTATGGCCACC | Start: 1773 |
| Tm | 59.963 | 59.891 | End: 1907 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
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| Sequence | TGCACGACTCCAGAAAACCA | ACTTTGTTTCCCTGCCACCA | Start: 1085 |
| Tm | 59.821 | 60.033 | End: 1246 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
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| Sequence | AGGCACACTTTTCCCCCTTT | ATAGTGGTGTGTGGTGCTGG | Start: 1651 |
| Tm | 59.735 | 59.963 | End: 1792 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
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| Sequence | AAGGCACACTTTTCCCCCTT | ATAGTGGTGTGTGGTGCTGG | Start: 1650 |
| Tm | 59.735 | 59.963 | End: 1792 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
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| Sequence | GAACGCAGGTCAAAAGGCAC | ATAGTGGTGTGTGGTGCTGG | Start: 1637 |
| Tm | 60.318 | 59.963 | End: 1792 |
| Size | 20 | 20 | 156 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGCACCACACACCACTAT | GCTCTAAGGGTATGGCCACC | Start: 1773 |
| Tm | 59.963 | 59.891 | End: 1907 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | TGCACGACTCCAGAAAACCA | ACTTTGTTTCCCTGCCACCA | Start: 1085 |
| Tm | 59.821 | 60.033 | End: 1246 |
| Size | 20 | 20 | 162 |
| Primer 3 details |
|---|
| Sequence | AGGCACACTTTTCCCCCTTT | ATAGTGGTGTGTGGTGCTGG | Start: 1651 |
| Tm | 59.735 | 59.963 | End: 1792 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | AAGGCACACTTTTCCCCCTT | ATAGTGGTGTGTGGTGCTGG | Start: 1650 |
| Tm | 59.735 | 59.963 | End: 1792 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
|---|
| Sequence | GAACGCAGGTCAAAAGGCAC | ATAGTGGTGTGTGGTGCTGG | Start: 1637 |
| Tm | 60.318 | 59.963 | End: 1792 |
| Size | 20 | 20 | 156 |
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