ssr_primer
Primer Details for TRINITY_DN74578_c1_g2_i7 |
|---|
| (A)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAACGGACAGGTCACTAA | GCGTGAGTCAGGACAAGACA | Start: 627 |
| Tm | 59.966 | 59.968 | End: 824 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | AACTGTAGCCAACGGACAGG | GCGTGAGTCAGGACAAGACA | Start: 620 |
| Tm | 59.965 | 59.968 | End: 824 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
|---|
| Sequence | ACGTATGTCAGCCCGTTGTT | ATTGAAATGGCGGAGGAGCA | Start: 3306 |
| Tm | 59.966 | 60.034 | End: 3569 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | AGCATTGCCATCTCCAGCTT | ATCATCGGGGCAGCAATAGG | Start: 1900 |
| Tm | 60.033 | 59.963 | End: 2057 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | ACGAGGGCTTTCTCTGCTTC | ATTGAAATGGCGGAGGAGCA | Start: 3463 |
| Tm | 60.037 | 60.034 | End: 3569 |
| Size | 20 | 20 | 107 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAACGGACAGGTCACTAA | GCGTGAGTCAGGACAAGACA | Start: 627 |
| Tm | 59.966 | 59.968 | End: 824 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | AACTGTAGCCAACGGACAGG | GCGTGAGTCAGGACAAGACA | Start: 620 |
| Tm | 59.965 | 59.968 | End: 824 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
|---|
| Sequence | ACGTATGTCAGCCCGTTGTT | ATTGAAATGGCGGAGGAGCA | Start: 3306 |
| Tm | 59.966 | 60.034 | End: 3569 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | AGCATTGCCATCTCCAGCTT | ATCATCGGGGCAGCAATAGG | Start: 1900 |
| Tm | 60.033 | 59.963 | End: 2057 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
|---|
| Sequence | ACGAGGGCTTTCTCTGCTTC | ATTGAAATGGCGGAGGAGCA | Start: 3463 |
| Tm | 60.037 | 60.034 | End: 3569 |
| Size | 20 | 20 | 107 |
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