Primer Details for TRINITY_DN74559_c1_g1_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCAGGGATTTAAATAATACTGCA | TCATCTTTTCTCACTATAACGGCT | Start: 50 |
| Tm | 58.867 | 57.778 | End: 158 |
| Size | 25 | 24 | 109 |
| Primer 2 details |
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| Sequence | CTGCCAGGGATTTAAATAATACTGCA | TCATCTTTTCTCACTATAACGGCT | Start: 49 |
| Tm | 59.735 | 57.778 | End: 158 |
| Size | 26 | 24 | 110 |
| Primer 3 details |
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| Sequence | ACTGCCAGGGATTTAAATAATACTGC | TCATCTTTTCTCACTATAACGGCT | Start: 48 |
| Tm | 59.735 | 57.778 | End: 158 |
| Size | 26 | 24 | 111 |
| Primer 4 details |
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| Sequence | TGCCAGGGATTTAAATAATACTGCA | TCATCTTTTCTCACTATAACGGCTT | Start: 50 |
| Tm | 58.867 | 58.359 | End: 158 |
| Size | 25 | 25 | 109 |
| Primer 5 details |
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| Sequence | TGCCAGGGATTTAAATAATACTGCA | TTCATCTTTTCTCACTATAACGGCT | Start: 50 |
| Tm | 58.867 | 58.359 | End: 159 |
| Size | 25 | 25 | 110 |
