ssr_primer
Primer Details for TRINITY_DN74550_c5_g2_i1 |
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| (T)32: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAGACAGCTGGCTCCTTC | GAGACGGGCGGGCAATTATA | Start: 135 |
| Tm | 59.964 | 59.966 | End: 253 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
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| Sequence | ACAAGACAGCTGGCTCCTTC | GGCGGGCAATTATACGGGTA | Start: 135 |
| Tm | 59.964 | 59.965 | End: 247 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
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| Sequence | ACAAGACAGCTGGCTCCTTC | ACCTCTCCCCGATAGAGACG | Start: 135 |
| Tm | 59.964 | 59.891 | End: 267 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
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| Sequence | ACAAGACAGCTGGCTCCTTC | TTTTGCCGCAAGTATTCCGC | Start: 135 |
| Tm | 59.964 | 60.11 | End: 304 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
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| Sequence | CACAAGACAGCTGGCTCCTT | GAGACGGGCGGGCAATTATA | Start: 134 |
| Tm | 60.251 | 59.966 | End: 253 |
| Size | 20 | 20 | 120 |
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