ssr_primer
Primer Details for TRINITY_DN74549_c2_g1_i4 |
|---|
| (T)31: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCTTTGTTGGATGGTGTG | GCAACAGCCTCCATCTGTCT | Start: 305 |
| Tm | 60.039 | 60.036 | End: 542 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | GCTGTCTGGTCTGGCAATCT | CACACCATCCAACAAAGGCG | Start: 170 |
| Tm | 60.036 | 60.039 | End: 324 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | CGTCAGTGTTGCTCTGTTGC | AAGCGACTCTCAGGCCTCTA | Start: 722 |
| Tm | 60.042 | 60.034 | End: 969 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | GCGTCAGTGTTGCTCTGTTG | AAGCGACTCTCAGGCCTCTA | Start: 721 |
| Tm | 60.042 | 60.034 | End: 969 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TGCCTTCCACTGGAATGACC | CACACCATCCAACAAAGGCG | Start: 109 |
| Tm | 59.962 | 60.039 | End: 324 |
| Size | 20 | 20 | 216 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCCTTTGTTGGATGGTGTG | GCAACAGCCTCCATCTGTCT | Start: 305 |
| Tm | 60.039 | 60.036 | End: 542 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | GCTGTCTGGTCTGGCAATCT | CACACCATCCAACAAAGGCG | Start: 170 |
| Tm | 60.036 | 60.039 | End: 324 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | CGTCAGTGTTGCTCTGTTGC | AAGCGACTCTCAGGCCTCTA | Start: 722 |
| Tm | 60.042 | 60.034 | End: 969 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | GCGTCAGTGTTGCTCTGTTG | AAGCGACTCTCAGGCCTCTA | Start: 721 |
| Tm | 60.042 | 60.034 | End: 969 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | TGCCTTCCACTGGAATGACC | CACACCATCCAACAAAGGCG | Start: 109 |
| Tm | 59.962 | 60.039 | End: 324 |
| Size | 20 | 20 | 216 |
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