ssr_primer
Primer Details for TRINITY_DN74521_c1_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTTGTGAAGATTCGCCGA | TCTGCTTCACTGGTACGCTG | Start: 24 |
| Tm | 59.966 | 60.038 | End: 238 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | GACAGTGAAACGGGGAGAGG | CCTGTACTTGGCACAGCTCA | Start: 1125 |
| Tm | 60.037 | 59.965 | End: 1246 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | TTCCTTTCCTTGTCGGACCG | TCTGCTTCACTGGTACGCTG | Start: 4 |
| Tm | 59.966 | 60.038 | End: 238 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | ACGTCCATTAAGCTCGCCAA | AGAAGTTGCACAGGGATGGG | Start: 1854 |
| Tm | 60.037 | 59.961 | End: 2125 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | AACGTCCATTAAGCTCGCCA | AGAAGTTGCACAGGGATGGG | Start: 1853 |
| Tm | 60.037 | 59.961 | End: 2125 |
| Size | 20 | 20 | 273 |
(CT)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTTGTGAAGATTCGCCGA | TCTGCTTCACTGGTACGCTG | Start: 24 |
| Tm | 59.966 | 60.038 | End: 238 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | GACAGTGAAACGGGGAGAGG | CCTGTACTTGGCACAGCTCA | Start: 1125 |
| Tm | 60.037 | 59.965 | End: 1246 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | TTCCTTTCCTTGTCGGACCG | TCTGCTTCACTGGTACGCTG | Start: 4 |
| Tm | 59.966 | 60.038 | End: 238 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | ACGTCCATTAAGCTCGCCAA | AGAAGTTGCACAGGGATGGG | Start: 1854 |
| Tm | 60.037 | 59.961 | End: 2125 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | AACGTCCATTAAGCTCGCCA | AGAAGTTGCACAGGGATGGG | Start: 1853 |
| Tm | 60.037 | 59.961 | End: 2125 |
| Size | 20 | 20 | 273 |
(T)15aaataatcaccaagaattattctaggtggcggtagccaccccggcccaatcctaaaaaattccccctgaaaaatgacgagacctaatg(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTTGTGAAGATTCGCCGA | TCTGCTTCACTGGTACGCTG | Start: 24 |
| Tm | 59.966 | 60.038 | End: 238 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | GACAGTGAAACGGGGAGAGG | CCTGTACTTGGCACAGCTCA | Start: 1125 |
| Tm | 60.037 | 59.965 | End: 1246 |
| Size | 20 | 20 | 122 |
| Primer 3 details |
|---|
| Sequence | TTCCTTTCCTTGTCGGACCG | TCTGCTTCACTGGTACGCTG | Start: 4 |
| Tm | 59.966 | 60.038 | End: 238 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | ACGTCCATTAAGCTCGCCAA | AGAAGTTGCACAGGGATGGG | Start: 1854 |
| Tm | 60.037 | 59.961 | End: 2125 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
|---|
| Sequence | AACGTCCATTAAGCTCGCCA | AGAAGTTGCACAGGGATGGG | Start: 1853 |
| Tm | 60.037 | 59.961 | End: 2125 |
| Size | 20 | 20 | 273 |
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