ssr_primer
Primer Details for TRINITY_DN74479_c2_g2_i2 |
|---|
| (T)17: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGCCCCCAAATGATTTTT | GGGAGCGCCACTTAATAGCT | Start: 470 |
| Tm | 60.036 | 59.893 | End: 633 |
| Size | 20 | 20 | 164 |
| Primer 2 details |
|---|
| Sequence | GGCGCCCCCAAATGATTTTT | CTTGGCGGAAAATGGTGAGC | Start: 470 |
| Tm | 60.036 | 60.109 | End: 735 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | GGCGCCCCCAAATGATTTTT | GCTTGGCGGAAAATGGTGAG | Start: 470 |
| Tm | 60.036 | 60.109 | End: 736 |
| Size | 20 | 20 | 267 |
| Primer 4 details |
|---|
| Sequence | GGCGCCCCCAAATGATTTTT | TGGGAGCGCCACTTAATAGC | Start: 470 |
| Tm | 60.036 | 60.179 | End: 634 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GGTGTAAACAAGGGAGCGGA | TTTCCAGTGGCGGTGTGAAT | Start: 20 |
| Tm | 59.964 | 60.179 | End: 202 |
| Size | 20 | 20 | 183 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGCCCCCAAATGATTTTT | GGGAGCGCCACTTAATAGCT | Start: 470 |
| Tm | 60.036 | 59.893 | End: 633 |
| Size | 20 | 20 | 164 |
| Primer 2 details |
|---|
| Sequence | GGCGCCCCCAAATGATTTTT | CTTGGCGGAAAATGGTGAGC | Start: 470 |
| Tm | 60.036 | 60.109 | End: 735 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
|---|
| Sequence | GGCGCCCCCAAATGATTTTT | GCTTGGCGGAAAATGGTGAG | Start: 470 |
| Tm | 60.036 | 60.109 | End: 736 |
| Size | 20 | 20 | 267 |
| Primer 4 details |
|---|
| Sequence | GGCGCCCCCAAATGATTTTT | TGGGAGCGCCACTTAATAGC | Start: 470 |
| Tm | 60.036 | 60.179 | End: 634 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GGTGTAAACAAGGGAGCGGA | TTTCCAGTGGCGGTGTGAAT | Start: 20 |
| Tm | 59.964 | 60.179 | End: 202 |
| Size | 20 | 20 | 183 |
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