ssr_primer
Primer Details for TRINITY_DN74476_c1_g4_i8 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGGTTCATGGGTCCATCAA | TGGCGGTTGGGATTGCTAAT | Start: 2483 |
| Tm | 60.035 | 60.034 | End: 2679 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
|---|
| Sequence | TCGTATGGAATCGACTGGCG | CAGCTGGACGGCAAAACAAA | Start: 349 |
| Tm | 59.97 | 59.898 | End: 575 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
|---|
| Sequence | ATGATGCCCTCATGTTGCGA | CGCCAGTCGATTCCATACGA | Start: 237 |
| Tm | 60.107 | 59.97 | End: 368 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | ATGATGCCCTCATGTTGCGA | TCGCCAGTCGATTCCATACG | Start: 237 |
| Tm | 60.107 | 59.97 | End: 369 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | TCCCTGCTTGCCGTAACTTT | TTGATGGACCCATGAACCGG | Start: 2269 |
| Tm | 59.89 | 60.035 | End: 2502 |
| Size | 20 | 20 | 234 |
(C)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGGTTCATGGGTCCATCAA | TGGCGGTTGGGATTGCTAAT | Start: 2483 |
| Tm | 60.035 | 60.034 | End: 2679 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
|---|
| Sequence | TCGTATGGAATCGACTGGCG | CAGCTGGACGGCAAAACAAA | Start: 349 |
| Tm | 59.97 | 59.898 | End: 575 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
|---|
| Sequence | ATGATGCCCTCATGTTGCGA | CGCCAGTCGATTCCATACGA | Start: 237 |
| Tm | 60.107 | 59.97 | End: 368 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
|---|
| Sequence | ATGATGCCCTCATGTTGCGA | TCGCCAGTCGATTCCATACG | Start: 237 |
| Tm | 60.107 | 59.97 | End: 369 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
|---|
| Sequence | TCCCTGCTTGCCGTAACTTT | TTGATGGACCCATGAACCGG | Start: 2269 |
| Tm | 59.89 | 60.035 | End: 2502 |
| Size | 20 | 20 | 234 |
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