ssr_primer
Primer Details for TRINITY_DN74446_c5_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTGTAGTTCCGCCGTCGA | GAACTCCAGCCTATCGACCG | Start: 290 |
| Tm | 59.968 | 59.969 | End: 520 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | TTTTGTAGTTCCGCCGTCGA | CGGACTTTGAACTCCAGCCT | Start: 290 |
| Tm | 59.968 | 59.965 | End: 528 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | CGGCTTGTAACGTTGCAGAC | GAACTCCAGCCTATCGACCG | Start: 342 |
| Tm | 60.111 | 59.969 | End: 520 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | CGGCTTGTAACGTTGCAGAC | CGGACTTTGAACTCCAGCCT | Start: 342 |
| Tm | 60.111 | 59.965 | End: 528 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
|---|
| Sequence | TCCGGGGAAATTTGGCAACT | TATGTGGCTCACTGCTGCAA | Start: 5 |
| Tm | 60.179 | 59.963 | End: 193 |
| Size | 20 | 20 | 189 |
(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTGTAGTTCCGCCGTCGA | GAACTCCAGCCTATCGACCG | Start: 290 |
| Tm | 59.968 | 59.969 | End: 520 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | TTTTGTAGTTCCGCCGTCGA | CGGACTTTGAACTCCAGCCT | Start: 290 |
| Tm | 59.968 | 59.965 | End: 528 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | CGGCTTGTAACGTTGCAGAC | GAACTCCAGCCTATCGACCG | Start: 342 |
| Tm | 60.111 | 59.969 | End: 520 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | CGGCTTGTAACGTTGCAGAC | CGGACTTTGAACTCCAGCCT | Start: 342 |
| Tm | 60.111 | 59.965 | End: 528 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
|---|
| Sequence | TCCGGGGAAATTTGGCAACT | TATGTGGCTCACTGCTGCAA | Start: 5 |
| Tm | 60.179 | 59.963 | End: 193 |
| Size | 20 | 20 | 189 |
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