ssr_primer
Primer Details for TRINITY_DN74401_c5_g1_i1 |
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| (G)15ccggccaaagggaaagaggggca(G)10c(G)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGCGGATTTAGGTGAGCG | TTTTGCCGCAGTTTTCCTCG | Start: 122 |
| Tm | 59.897 | 59.97 | End: 327 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
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| Sequence | AGAGCGGATTTAGGTGAGCG | CTTTCCCGGTTCTCTGTGCT | Start: 122 |
| Tm | 59.897 | 59.965 | End: 401 |
| Size | 20 | 20 | 280 |
| Primer 3 details |
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| Sequence | AGAGCGGATTTAGGTGAGCG | GGTTCTCTGTGCTACTGCGT | Start: 122 |
| Tm | 59.897 | 60.038 | End: 394 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
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| Sequence | AGTCTCTCAGAGTGGGGCTT | TTTTGCCGCAGTTTTCCTCG | Start: 197 |
| Tm | 59.884 | 59.97 | End: 327 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
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| Sequence | AGTCTCTCAGAGTGGGGCTT | CTTTCCCGGTTCTCTGTGCT | Start: 197 |
| Tm | 59.884 | 59.965 | End: 401 |
| Size | 20 | 20 | 205 |
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