ssr_primer
Primer Details for TRINITY_DN74387_c2_g3_i4 |
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| (G)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGCCCTGCTCTTTCTCCTT | AGGGAGGATCTGAAAGGGCT | Start: 2327 |
| Tm | 59.963 | 59.955 | End: 2570 |
| Size | 20 | 20 | 244 |
| Primer 2 details |
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| Sequence | GCGTCCTAATGGTCAAACGC | GCTGCTTGAGCGCCTCTATA | Start: 1182 |
| Tm | 59.904 | 59.967 | End: 1445 |
| Size | 20 | 20 | 264 |
| Primer 3 details |
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| Sequence | ATCTCAAACGGAGCTAGGCG | CCTGTTGGGCTCTAGTTGGG | Start: 604 |
| Tm | 59.897 | 60.035 | End: 782 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
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| Sequence | ATCTCAAACGGAGCTAGGCG | TTGGGCTCTAGTTGGGCTTG | Start: 604 |
| Tm | 59.897 | 59.962 | End: 778 |
| Size | 20 | 20 | 175 |
| Primer 5 details |
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| Sequence | ATCTCAAACGGAGCTAGGCG | GTTGGGCTCTAGTTGGGCTT | Start: 604 |
| Tm | 59.897 | 59.962 | End: 779 |
| Size | 20 | 20 | 176 |
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