ssr_primer
Primer Details for TRINITY_DN74375_c9_g5_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGGAGTTTTGCGACGTATC | AGGAGCCCTATCGTCGATGA | Start: 162 |
| Tm | 59.975 | 59.889 | End: 301 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
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| Sequence | AGAACCAAATTCCGCGGAGT | AGGAGCCCTATCGTCGATGA | Start: 149 |
| Tm | 59.964 | 59.889 | End: 301 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
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| Sequence | ACAAGATGCGCAGAAAGGGA | AGGAGCCCTATCGTCGATGA | Start: 90 |
| Tm | 59.963 | 59.889 | End: 301 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
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| Sequence | GACATCAGCAAATGCCACCC | GATACGTCGCAAAACTCCGC | Start: 38 |
| Tm | 59.826 | 59.975 | End: 181 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
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| Sequence | GCGGAGTTTTGCGACGTATC | TGGAGACGAGGAGCCCTATC | Start: 162 |
| Tm | 59.975 | 60.179 | End: 309 |
| Size | 20 | 20 | 148 |
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