Primer Details for TRINITY_DN74366_c7_g1_i1 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAATATCGCCAGATTTCATTCGA | AGCGCATCCGACCCATTAAA | Start: 17 |
| Tm | 58.458 | 60.107 | End: 123 |
| Size | 24 | 20 | 107 |
| Primer 2 details | |||
| Sequence | GAATATCGCCAGATTTCATTCGA | AGCGCATCCGACCCATTAAA | Start: 18 |
| Tm | 57.154 | 60.107 | End: 123 |
| Size | 23 | 20 | 106 |
| Primer 3 details | |||
| Sequence | AGAATATCGCCAGATTTCATTCG | AGCGCATCCGACCCATTAAA | Start: 17 |
| Tm | 57.147 | 60.107 | End: 123 |
| Size | 23 | 20 | 107 |
| Primer 4 details | |||
| Sequence | TCGCCAGATTTCATTCGATAAAATGT | AGCGCATCCGACCCATTAAA | Start: 23 |
| Tm | 59.962 | 60.107 | End: 123 |
| Size | 26 | 20 | 101 |
| Primer 5 details | |||
| Sequence | AGAATATCGCCAGATTTCATTCGA | ATTTTGCCACCCTGTTCTGC | Start: 17 |
| Tm | 58.458 | 59.319 | End: 147 |
| Size | 24 | 20 | 131 |
