ssr_primer
Primer Details for TRINITY_DN74362_c1_g1_i3 |
|---|
| (T)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTTTTGTCGGCATAGGCT | GCAAATACGGCGCATGAGTT | Start: 983 |
| Tm | 60.037 | 59.901 | End: 1217 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | GTCCAGGTTGTTCCCGGAAT | TTGGGGCAGGCCTGTAAAAA | Start: 2045 |
| Tm | 59.962 | 60.105 | End: 2270 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | GTGTTGAGGAGGAAGGGCAA | AGCCTATGCCGACAAAACGA | Start: 832 |
| Tm | 59.89 | 60.037 | End: 1002 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | GTCCAGGTTGTTCCCGGAAT | TGGGGCAGGCCTGTAAAAAT | Start: 2045 |
| Tm | 59.962 | 59.884 | End: 2269 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | GTCCAGGTTGTTCCCGGAAT | TCACCTTCCCATCCCTGAGT | Start: 2045 |
| Tm | 59.962 | 59.881 | End: 2241 |
| Size | 20 | 20 | 197 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTTTTGTCGGCATAGGCT | GCAAATACGGCGCATGAGTT | Start: 983 |
| Tm | 60.037 | 59.901 | End: 1217 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | GTCCAGGTTGTTCCCGGAAT | TTGGGGCAGGCCTGTAAAAA | Start: 2045 |
| Tm | 59.962 | 60.105 | End: 2270 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | GTGTTGAGGAGGAAGGGCAA | AGCCTATGCCGACAAAACGA | Start: 832 |
| Tm | 59.89 | 60.037 | End: 1002 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | GTCCAGGTTGTTCCCGGAAT | TGGGGCAGGCCTGTAAAAAT | Start: 2045 |
| Tm | 59.962 | 59.884 | End: 2269 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | GTCCAGGTTGTTCCCGGAAT | TCACCTTCCCATCCCTGAGT | Start: 2045 |
| Tm | 59.962 | 59.881 | End: 2241 |
| Size | 20 | 20 | 197 |
(A)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTTTTGTCGGCATAGGCT | GCAAATACGGCGCATGAGTT | Start: 983 |
| Tm | 60.037 | 59.901 | End: 1217 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
|---|
| Sequence | GTCCAGGTTGTTCCCGGAAT | TTGGGGCAGGCCTGTAAAAA | Start: 2045 |
| Tm | 59.962 | 60.105 | End: 2270 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | GTGTTGAGGAGGAAGGGCAA | AGCCTATGCCGACAAAACGA | Start: 832 |
| Tm | 59.89 | 60.037 | End: 1002 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | GTCCAGGTTGTTCCCGGAAT | TGGGGCAGGCCTGTAAAAAT | Start: 2045 |
| Tm | 59.962 | 59.884 | End: 2269 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | GTCCAGGTTGTTCCCGGAAT | TCACCTTCCCATCCCTGAGT | Start: 2045 |
| Tm | 59.962 | 59.881 | End: 2241 |
| Size | 20 | 20 | 197 |
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