ssr_primer
Primer Details for TRINITY_DN74303_c3_g1_i2 |
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| (G)37: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCGCCATGGTCATCGATT | GCAGGGGCTATCGTCCTTTT | Start: 106 |
| Tm | 60.25 | 60.107 | End: 251 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
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| Sequence | AGGGTTGGAGGATAGTGGGG | GCAGGGGCTATCGTCCTTTT | Start: 17 |
| Tm | 60.328 | 60.107 | End: 251 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
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| Sequence | AGGGTTGGAGGATAGTGGGG | TGACCCAATCGATGCGATGT | Start: 17 |
| Tm | 60.328 | 59.822 | End: 199 |
| Size | 20 | 20 | 183 |
| Primer 4 details |
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| Sequence | AGGGTTGGAGGATAGTGGGG | ATGCCAGTTCAACCACGTCA | Start: 17 |
| Tm | 60.328 | 60.179 | End: 174 |
| Size | 20 | 20 | 158 |
| Primer 5 details |
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| Sequence | AGGGTTGGAGGATAGTGGGG | CATGCCAGTTCAACCACGTC | Start: 17 |
| Tm | 60.328 | 59.762 | End: 175 |
| Size | 20 | 20 | 159 |
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