ssr_primer
Primer Details for TRINITY_DN74302_c3_g3_i4 |
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| (C)13aacaaattttgtgtgaattttgaacatgagtcggatttgcactgaatttttcatagtattttggagaaaaaatata(C)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGGGGTTAGGAAGGCACC | GGTGTGCATGTAGAGGCCAT | Start: 7 |
| Tm | 59.665 | 60.107 | End: 197 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
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| Sequence | ATTGGGGTTAGGAAGGCACC | TCTGGTGTGCATGTAGAGGC | Start: 7 |
| Tm | 59.665 | 59.75 | End: 200 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
|---|
| Sequence | ACGTTGTTTGTGTGCGTTCT | ATACTGTTTTCCGGCCCCAG | Start: 416 |
| Tm | 59.2 | 60.034 | End: 565 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
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| Sequence | ACGTTGTTTGTGTGCGTTCT | GATACTGTTTTCCGGCCCCA | Start: 416 |
| Tm | 59.2 | 60.035 | End: 566 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | ATGGCCTCTACATGCACACC | AGAACGCACACAAACAACGT | Start: 178 |
| Tm | 60.107 | 59.2 | End: 435 |
| Size | 20 | 20 | 258 |
(C)18(TC)6ctttttattctttacatttctctctatttttttgtttgttttc(T)30: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGGGGTTAGGAAGGCACC | GGTGTGCATGTAGAGGCCAT | Start: 7 |
| Tm | 59.665 | 60.107 | End: 197 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | ATTGGGGTTAGGAAGGCACC | TCTGGTGTGCATGTAGAGGC | Start: 7 |
| Tm | 59.665 | 59.75 | End: 200 |
| Size | 20 | 20 | 194 |
| Primer 3 details |
|---|
| Sequence | ACGTTGTTTGTGTGCGTTCT | ATACTGTTTTCCGGCCCCAG | Start: 416 |
| Tm | 59.2 | 60.034 | End: 565 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | ACGTTGTTTGTGTGCGTTCT | GATACTGTTTTCCGGCCCCA | Start: 416 |
| Tm | 59.2 | 60.035 | End: 566 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | ATGGCCTCTACATGCACACC | AGAACGCACACAAACAACGT | Start: 178 |
| Tm | 60.107 | 59.2 | End: 435 |
| Size | 20 | 20 | 258 |
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