ssr_primer
Primer Details for TRINITY_DN74217_c2_g1_i6 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCTTGCTCCCATTGCCTCA | AGCCGACATCAACGACATGT | Start: 1395 |
| Tm | 60.035 | 60.037 | End: 1660 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
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| Sequence | GGAGAGGTGCAGGAAGTTCC | GGGGAGCAGAAACTTTCCGA | Start: 1913 |
| Tm | 60.036 | 59.964 | End: 2179 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
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| Sequence | GGAGAGGTGCAGGAAGTTCC | AGGGGAGCAGAAACTTTCCG | Start: 1913 |
| Tm | 60.036 | 59.963 | End: 2180 |
| Size | 20 | 20 | 268 |
| Primer 4 details |
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| Sequence | GCGTCGCTTGATTCTAGGGA | TGAGGCAATGGGAGCAAGAG | Start: 1313 |
| Tm | 59.898 | 60.035 | End: 1414 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
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| Sequence | AGCGTCGCTTGATTCTAGGG | TGAGGCAATGGGAGCAAGAG | Start: 1312 |
| Tm | 59.897 | 60.035 | End: 1414 |
| Size | 20 | 20 | 103 |
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