ssr_primer
Primer Details for TRINITY_DN74184_c0_g2_i4 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGCCTAGACTGTTGCTGGT | TCACATCCACGTGTCCCAAG | Start: 1033 |
| Tm | 59.965 | 59.965 | End: 1245 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
|---|
| Sequence | ACACACAGCGCATCGACTAA | TGTCATGGACACGGGAACTG | Start: 2149 |
| Tm | 60.039 | 59.965 | End: 2298 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
|---|
| Sequence | GTCCACTGTCGCAATGGAGA | ATCAGTGGCACCATCGTGTT | Start: 3923 |
| Tm | 60.037 | 59.963 | End: 4029 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | GTCCACTGTCGCAATGGAGA | AATCAGTGGCACCATCGTGT | Start: 3923 |
| Tm | 60.037 | 59.963 | End: 4030 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | CACCAAGGCCACTTCCAGAT | ATCAGTGGCACCATCGTGTT | Start: 3806 |
| Tm | 59.961 | 59.963 | End: 4029 |
| Size | 20 | 20 | 224 |
(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGCCTAGACTGTTGCTGGT | TCACATCCACGTGTCCCAAG | Start: 1033 |
| Tm | 59.965 | 59.965 | End: 1245 |
| Size | 20 | 20 | 213 |
| Primer 2 details |
|---|
| Sequence | ACACACAGCGCATCGACTAA | TGTCATGGACACGGGAACTG | Start: 2149 |
| Tm | 60.039 | 59.965 | End: 2298 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
|---|
| Sequence | GTCCACTGTCGCAATGGAGA | ATCAGTGGCACCATCGTGTT | Start: 3923 |
| Tm | 60.037 | 59.963 | End: 4029 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | GTCCACTGTCGCAATGGAGA | AATCAGTGGCACCATCGTGT | Start: 3923 |
| Tm | 60.037 | 59.963 | End: 4030 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | CACCAAGGCCACTTCCAGAT | ATCAGTGGCACCATCGTGTT | Start: 3806 |
| Tm | 59.961 | 59.963 | End: 4029 |
| Size | 20 | 20 | 224 |
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