ssr_primer
Primer Details for TRINITY_DN74144_c3_g1_i4 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAACGAGAACTCACGGTCC | GTGACTTGATTCCGCGATGC | Start: 418 |
| Tm | 59.968 | 59.973 | End: 597 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | GCATCGCGGAATCAAGTCAC | CGTGGCTTGTTTGGATGCAA | Start: 578 |
| Tm | 59.973 | 59.968 | End: 748 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | AGAACTCACGGTCCGAAACC | GTGACTTGATTCCGCGATGC | Start: 424 |
| Tm | 59.967 | 59.973 | End: 597 |
| Size | 20 | 20 | 174 |
| Primer 4 details |
|---|
| Sequence | GCATCGCGGAATCAAGTCAC | AGTAATTTCCGCCGGTCGTT | Start: 578 |
| Tm | 59.973 | 60.037 | End: 833 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | AACGACCGGCGGAAATTACT | CGTTTTTCTTCAGTGCGGCA | Start: 814 |
| Tm | 60.037 | 59.971 | End: 1026 |
| Size | 20 | 20 | 213 |
(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAACGAGAACTCACGGTCC | GTGACTTGATTCCGCGATGC | Start: 418 |
| Tm | 59.968 | 59.973 | End: 597 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | GCATCGCGGAATCAAGTCAC | CGTGGCTTGTTTGGATGCAA | Start: 578 |
| Tm | 59.973 | 59.968 | End: 748 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | AGAACTCACGGTCCGAAACC | GTGACTTGATTCCGCGATGC | Start: 424 |
| Tm | 59.967 | 59.973 | End: 597 |
| Size | 20 | 20 | 174 |
| Primer 4 details |
|---|
| Sequence | GCATCGCGGAATCAAGTCAC | AGTAATTTCCGCCGGTCGTT | Start: 578 |
| Tm | 59.973 | 60.037 | End: 833 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
|---|
| Sequence | AACGACCGGCGGAAATTACT | CGTTTTTCTTCAGTGCGGCA | Start: 814 |
| Tm | 60.037 | 59.971 | End: 1026 |
| Size | 20 | 20 | 213 |
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