ssr_primer
Primer Details for TRINITY_DN74116_c3_g3_i3 |
|---|
| (CT)10ccccctaaatccccttc(A)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACCTCGTAACCCCCTCC | TCCACCGAAGATAGGCAGGA | Start: 339 |
| Tm | 60.036 | 60.033 | End: 560 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | CAAACCTCGTAACCCCCTCC | AAACCCCGCTATGACAACGT | Start: 339 |
| Tm | 60.036 | 59.964 | End: 579 |
| Size | 20 | 20 | 241 |
| Primer 3 details |
|---|
| Sequence | CGGCGACCAGAATATCCCTC | GGTTGATGGTCGGTGCAGTA | Start: 1527 |
| Tm | 60.038 | 60.037 | End: 1694 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | ATGAGTGACAGGAGACCGGA | GGAGGGGGTTACGAGGTTTG | Start: 253 |
| Tm | 59.96 | 60.036 | End: 358 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | TTACAAAGGCGGTCCGAGAC | GAGGGATATTCTGGTCGCCG | Start: 1390 |
| Tm | 60.038 | 60.038 | End: 1546 |
| Size | 20 | 20 | 157 |
(A)28: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACCTCGTAACCCCCTCC | TCCACCGAAGATAGGCAGGA | Start: 339 |
| Tm | 60.036 | 60.033 | End: 560 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | CAAACCTCGTAACCCCCTCC | AAACCCCGCTATGACAACGT | Start: 339 |
| Tm | 60.036 | 59.964 | End: 579 |
| Size | 20 | 20 | 241 |
| Primer 3 details |
|---|
| Sequence | CGGCGACCAGAATATCCCTC | GGTTGATGGTCGGTGCAGTA | Start: 1527 |
| Tm | 60.038 | 60.037 | End: 1694 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | ATGAGTGACAGGAGACCGGA | GGAGGGGGTTACGAGGTTTG | Start: 253 |
| Tm | 59.96 | 60.036 | End: 358 |
| Size | 20 | 20 | 106 |
| Primer 5 details |
|---|
| Sequence | TTACAAAGGCGGTCCGAGAC | GAGGGATATTCTGGTCGCCG | Start: 1390 |
| Tm | 60.038 | 60.038 | End: 1546 |
| Size | 20 | 20 | 157 |
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