ssr_primer
Primer Details for TRINITY_DN74111_c4_g1_i2 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGACTGAGCGCTAACTTGT | TGTGGAGGTGGAAAAGACGG | Start: 154 |
| Tm | 60.037 | 59.892 | End: 330 |
| Size | 20 | 20 | 177 |
| Primer 2 details |
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| Sequence | CCGTCTTTTCCACCTCCACA | CCTGGGTAAGAAAGCGACGT | Start: 311 |
| Tm | 59.892 | 60.038 | End: 513 |
| Size | 20 | 20 | 203 |
| Primer 3 details |
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| Sequence | TGCTCGTGGAAGCTGGATAC | ACAAGTTAGCGCTCAGTCCC | Start: 64 |
| Tm | 59.824 | 60.037 | End: 173 |
| Size | 20 | 20 | 110 |
| Primer 4 details |
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| Sequence | GTGCTCGTGGAAGCTGGATA | ACAAGTTAGCGCTCAGTCCC | Start: 63 |
| Tm | 59.824 | 60.037 | End: 173 |
| Size | 20 | 20 | 111 |
| Primer 5 details |
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| Sequence | CCGTCTTTTCCACCTCCACA | CTCCCCTGGGTAAGAAAGCG | Start: 311 |
| Tm | 59.892 | 60.108 | End: 517 |
| Size | 20 | 20 | 207 |
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