ssr_primer
Primer Details for TRINITY_DN74062_c4_g1_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCACTGGATTGTGGGGAA | CCATCCCGTCCGGAAGAAAT | Start: 800 |
| Tm | 59.813 | 59.82 | End: 1069 |
| Size | 20 | 20 | 270 |
| Primer 2 details |
|---|
| Sequence | GCGAACCCTCACCACAATTG | CGTAGATGCGGAACAGTGGA | Start: 1138 |
| Tm | 59.759 | 59.827 | End: 1384 |
| Size | 20 | 20 | 247 |
| Primer 3 details |
|---|
| Sequence | GCGAACCCTCACCACAATTG | TCGTAGATGCGGAACAGTGG | Start: 1138 |
| Tm | 59.759 | 59.827 | End: 1385 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | ATTTCTTCCGGACGGGATGG | CAATTGTGGTGAGGGTTCGC | Start: 1050 |
| Tm | 59.82 | 59.759 | End: 1157 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | CGCCGAGAATTGATGGCCTA | AATGCGGAGAGTATGGTGCC | Start: 77 |
| Tm | 60.25 | 60.179 | End: 184 |
| Size | 20 | 20 | 108 |
(A)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCACTGGATTGTGGGGAA | CCATCCCGTCCGGAAGAAAT | Start: 800 |
| Tm | 59.813 | 59.82 | End: 1069 |
| Size | 20 | 20 | 270 |
| Primer 2 details |
|---|
| Sequence | GCGAACCCTCACCACAATTG | CGTAGATGCGGAACAGTGGA | Start: 1138 |
| Tm | 59.759 | 59.827 | End: 1384 |
| Size | 20 | 20 | 247 |
| Primer 3 details |
|---|
| Sequence | GCGAACCCTCACCACAATTG | TCGTAGATGCGGAACAGTGG | Start: 1138 |
| Tm | 59.759 | 59.827 | End: 1385 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | ATTTCTTCCGGACGGGATGG | CAATTGTGGTGAGGGTTCGC | Start: 1050 |
| Tm | 59.82 | 59.759 | End: 1157 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | CGCCGAGAATTGATGGCCTA | AATGCGGAGAGTATGGTGCC | Start: 77 |
| Tm | 60.25 | 60.179 | End: 184 |
| Size | 20 | 20 | 108 |
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