ssr_primer
Primer Details for TRINITY_DN73960_c0_g1_i9 |
|---|
| (A)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAACTTGCCGCGTTGTTTA | GAGCGTGAGAGAGCATCCTC | Start: 2574 |
| Tm | 59.97 | 59.969 | End: 2787 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | GATTTGGCGACCATCATGGC | TGGAACGGAGAGCTCACAAC | Start: 788 |
| Tm | 59.969 | 59.966 | End: 1022 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | TAACCAACCCTGACACTCGC | GCCATGATGGTCGCCAAATC | Start: 658 |
| Tm | 59.966 | 59.969 | End: 807 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | GAGGATGCTCTCTCACGCTC | CACTCCCAAACCGTCATCCA | Start: 2768 |
| Tm | 59.969 | 59.964 | End: 2912 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | CCTGACACTCGCATTGACCT | GCCATGATGGTCGCCAAATC | Start: 666 |
| Tm | 60.037 | 59.969 | End: 807 |
| Size | 20 | 20 | 142 |
(A)32: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAACTTGCCGCGTTGTTTA | GAGCGTGAGAGAGCATCCTC | Start: 2574 |
| Tm | 59.97 | 59.969 | End: 2787 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | GATTTGGCGACCATCATGGC | TGGAACGGAGAGCTCACAAC | Start: 788 |
| Tm | 59.969 | 59.966 | End: 1022 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
|---|
| Sequence | TAACCAACCCTGACACTCGC | GCCATGATGGTCGCCAAATC | Start: 658 |
| Tm | 59.966 | 59.969 | End: 807 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | GAGGATGCTCTCTCACGCTC | CACTCCCAAACCGTCATCCA | Start: 2768 |
| Tm | 59.969 | 59.964 | End: 2912 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | CCTGACACTCGCATTGACCT | GCCATGATGGTCGCCAAATC | Start: 666 |
| Tm | 60.037 | 59.969 | End: 807 |
| Size | 20 | 20 | 142 |
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