Primer Details for TRINITY_DN73953_c10_g1_i1 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCCTTCTTTCTTGCCTCT | TGATCTTCAAGTTCAAAATGATGCA | Start: 58 |
| Tm | 58.374 | 58.016 | End: 157 |
| Size | 21 | 25 | 100 |
| Primer 2 details |
|---|
| Sequence | TCCTCCTTCTTTCTTGCCTCT | AGTTGAGAACACAAAAATAGCTTGA | Start: 58 |
| Tm | 58.374 | 57.666 | End: 179 |
| Size | 21 | 25 | 122 |
| Primer 3 details |
|---|
| Sequence | TCCTCCTTCTTTCTTGCCTCT | TGAAGTTGAGAACACAAAAATAGCT | Start: 58 |
| Tm | 58.374 | 57.666 | End: 182 |
| Size | 21 | 25 | 125 |
| Primer 4 details |
|---|
| Sequence | TCCTCCTTCTTTCTTGCCTCTT | TGATCTTCAAGTTCAAAATGATGCA | Start: 58 |
| Tm | 59.018 | 58.016 | End: 157 |
| Size | 22 | 25 | 100 |
| Primer 5 details |
|---|
| Sequence | TTCCTCCTTCTTTCTTGCCTCT | TGATCTTCAAGTTCAAAATGATGCA | Start: 57 |
| Tm | 59.018 | 58.016 | End: 157 |
| Size | 22 | 25 | 101 |
