ssr_primer
Primer Details for TRINITY_DN73926_c5_g2_i2 |
|---|
| (T)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCGTCCTCGAGTTTTGGT | TCTCTGTTTTCCAGACCGCC | Start: 185 |
| Tm | 59.967 | 59.965 | End: 305 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
|---|
| Sequence | TTGGTCCCCGAGAGCTTTTC | TCTCTGTTTTCCAGACCGCC | Start: 200 |
| Tm | 59.964 | 59.965 | End: 305 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
|---|
| Sequence | CCGTTGCCAGAGCTAACAGA | TCTCTGTTTTCCAGACCGCC | Start: 137 |
| Tm | 60.037 | 59.965 | End: 305 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
|---|
| Sequence | AACGTATGCCGCGATAACCT | ACCAAAACTCGAGGACGGAC | Start: 52 |
| Tm | 59.896 | 59.967 | End: 204 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
|---|
| Sequence | AACGTATGCCGCGATAACCT | TCTCTGTTTTCCAGACCGCC | Start: 52 |
| Tm | 59.896 | 59.965 | End: 305 |
| Size | 20 | 20 | 254 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCCGTCCTCGAGTTTTGGT | TCTCTGTTTTCCAGACCGCC | Start: 185 |
| Tm | 59.967 | 59.965 | End: 305 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
|---|
| Sequence | TTGGTCCCCGAGAGCTTTTC | TCTCTGTTTTCCAGACCGCC | Start: 200 |
| Tm | 59.964 | 59.965 | End: 305 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
|---|
| Sequence | CCGTTGCCAGAGCTAACAGA | TCTCTGTTTTCCAGACCGCC | Start: 137 |
| Tm | 60.037 | 59.965 | End: 305 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
|---|
| Sequence | AACGTATGCCGCGATAACCT | ACCAAAACTCGAGGACGGAC | Start: 52 |
| Tm | 59.896 | 59.967 | End: 204 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
|---|
| Sequence | AACGTATGCCGCGATAACCT | TCTCTGTTTTCCAGACCGCC | Start: 52 |
| Tm | 59.896 | 59.965 | End: 305 |
| Size | 20 | 20 | 254 |
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