ssr_primer
Primer Details for TRINITY_DN73911_c2_g1_i13 |
|---|
| (A)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATCCACTGGTTGGCAAAG | TGTTGTGAGGGCCCAAAGTT | Start: 2454 |
| Tm | 60.038 | 60.033 | End: 2618 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
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| Sequence | GCATCCACTGGTTGGCAAAG | CTCACAGCCAGTCTCTTGCA | Start: 2454 |
| Tm | 60.038 | 59.966 | End: 2564 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | TGCTTGGGGGCAACATTTTG | ATCCCAACAGCCTCCAATGG | Start: 644 |
| Tm | 59.892 | 60.033 | End: 829 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GCTTGGGGGCAACATTTTGT | ATCCCAACAGCCTCCAATGG | Start: 645 |
| Tm | 59.892 | 60.033 | End: 829 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | AACTTTGGGCCCTCACAACA | CTCCCGCAATGTTTTGTCCG | Start: 2599 |
| Tm | 60.033 | 60.11 | End: 2712 |
| Size | 20 | 20 | 114 |
(C)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATCCACTGGTTGGCAAAG | TGTTGTGAGGGCCCAAAGTT | Start: 2454 |
| Tm | 60.038 | 60.033 | End: 2618 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | GCATCCACTGGTTGGCAAAG | CTCACAGCCAGTCTCTTGCA | Start: 2454 |
| Tm | 60.038 | 59.966 | End: 2564 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | TGCTTGGGGGCAACATTTTG | ATCCCAACAGCCTCCAATGG | Start: 644 |
| Tm | 59.892 | 60.033 | End: 829 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GCTTGGGGGCAACATTTTGT | ATCCCAACAGCCTCCAATGG | Start: 645 |
| Tm | 59.892 | 60.033 | End: 829 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | AACTTTGGGCCCTCACAACA | CTCCCGCAATGTTTTGTCCG | Start: 2599 |
| Tm | 60.033 | 60.11 | End: 2712 |
| Size | 20 | 20 | 114 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATCCACTGGTTGGCAAAG | TGTTGTGAGGGCCCAAAGTT | Start: 2454 |
| Tm | 60.038 | 60.033 | End: 2618 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | GCATCCACTGGTTGGCAAAG | CTCACAGCCAGTCTCTTGCA | Start: 2454 |
| Tm | 60.038 | 59.966 | End: 2564 |
| Size | 20 | 20 | 111 |
| Primer 3 details |
|---|
| Sequence | TGCTTGGGGGCAACATTTTG | ATCCCAACAGCCTCCAATGG | Start: 644 |
| Tm | 59.892 | 60.033 | End: 829 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GCTTGGGGGCAACATTTTGT | ATCCCAACAGCCTCCAATGG | Start: 645 |
| Tm | 59.892 | 60.033 | End: 829 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | AACTTTGGGCCCTCACAACA | CTCCCGCAATGTTTTGTCCG | Start: 2599 |
| Tm | 60.033 | 60.11 | End: 2712 |
| Size | 20 | 20 | 114 |
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