ssr_primer
Primer Details for TRINITY_DN73911_c1_g1_i2 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAACGTCCGCCATTTTTGG | ATGCCAAGAACTCCACGAGG | Start: 130 |
| Tm | 59.969 | 60.036 | End: 385 |
| Size | 20 | 20 | 256 |
| Primer 2 details |
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| Sequence | CCTCGTGGAGTTCTTGGCAT | TAGAGCTGGGGGAGTCTTCC | Start: 366 |
| Tm | 60.036 | 60.032 | End: 483 |
| Size | 20 | 20 | 118 |
| Primer 3 details |
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| Sequence | CCTCGTGGAGTTCTTGGCAT | CCCCCGAGTCCTCCTTTAGA | Start: 366 |
| Tm | 60.036 | 60.032 | End: 499 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
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| Sequence | CCTCGTGGAGTTCTTGGCAT | TCCCCCGAGTCCTCCTTTAG | Start: 366 |
| Tm | 60.036 | 60.032 | End: 500 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
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| Sequence | TCCGGACAGCCTCTTGAAAC | ATGCCAAGAACTCCACGAGG | Start: 153 |
| Tm | 59.965 | 60.036 | End: 385 |
| Size | 20 | 20 | 233 |
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