ssr_primer
Primer Details for TRINITY_DN73876_c0_g1_i1 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAAAAGAACCAGCGCGAT | CGATTTCCGGGTCTGGATGT | Start: 25 |
| Tm | 59.834 | 59.822 | End: 275 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | AGAACCAGCGCGATTACAGT | CGATTTCCGGGTCTGGATGT | Start: 31 |
| Tm | 59.753 | 59.822 | End: 275 |
| Size | 20 | 20 | 245 |
| Primer 3 details |
|---|
| Sequence | CGAAAAAGAACCAGCGCGAT | ACCAAAATCGCTTCCGCTCT | Start: 25 |
| Tm | 59.834 | 60.322 | End: 151 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | ACCAGCGCGATTACAGTGTT | CGATTTCCGGGTCTGGATGT | Start: 34 |
| Tm | 60.32 | 59.822 | End: 275 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | AACCAGCGCGATTACAGTGT | CGATTTCCGGGTCTGGATGT | Start: 33 |
| Tm | 60.32 | 59.822 | End: 275 |
| Size | 20 | 20 | 243 |
(T)11actttca(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAAAAGAACCAGCGCGAT | CGATTTCCGGGTCTGGATGT | Start: 25 |
| Tm | 59.834 | 59.822 | End: 275 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
|---|
| Sequence | AGAACCAGCGCGATTACAGT | CGATTTCCGGGTCTGGATGT | Start: 31 |
| Tm | 59.753 | 59.822 | End: 275 |
| Size | 20 | 20 | 245 |
| Primer 3 details |
|---|
| Sequence | CGAAAAAGAACCAGCGCGAT | ACCAAAATCGCTTCCGCTCT | Start: 25 |
| Tm | 59.834 | 60.322 | End: 151 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | ACCAGCGCGATTACAGTGTT | CGATTTCCGGGTCTGGATGT | Start: 34 |
| Tm | 60.32 | 59.822 | End: 275 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | AACCAGCGCGATTACAGTGT | CGATTTCCGGGTCTGGATGT | Start: 33 |
| Tm | 60.32 | 59.822 | End: 275 |
| Size | 20 | 20 | 243 |
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