ssr_primer
Primer Details for TRINITY_DN73783_c5_g1_i1 |
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| (A)16gggaaagaatgtaagaaaaataagaacagaaaaaaggaaaaaaagaaaagacaagacagaaagaatag(A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGAAGTTGCCTTTCCTCACT | CCGATCTAGAGGACGAGAAGAG | Start: 141 |
| Tm | 59.56 | 58.936 | End: 240 |
| Size | 22 | 22 | 100 |
| Primer 2 details |
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| Sequence | TCTGAAGTTGCCTTTCCTCACT | CCGATCTAGAGGACGAGAAGA | Start: 141 |
| Tm | 59.56 | 57.885 | End: 240 |
| Size | 22 | 21 | 100 |
| Primer 3 details |
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| Sequence | TCTGAAGTTGCCTTTCCTCAC | CCGATCTAGAGGACGAGAAGAG | Start: 141 |
| Tm | 58.149 | 58.936 | End: 240 |
| Size | 21 | 22 | 100 |
| Primer 4 details |
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| Sequence | TCTGAAGTTGCCTTTCCTCAC | CCGATCTAGAGGACGAGAAGA | Start: 141 |
| Tm | 58.149 | 57.885 | End: 240 |
| Size | 21 | 21 | 100 |
| Primer 5 details |
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| Sequence | TTCTGAAGTTGCCTTTCCTCACT | CGATCTAGAGGACGAGAAGAGG | Start: 140 |
| Tm | 60.118 | 58.936 | End: 239 |
| Size | 23 | 22 | 100 |
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